Volumetric absorptive microsampling as alternative sampling technique for renal function assessment in the paediatric population using iohexol

The glomerular filtration rate (GFR) is considered the best overall index for renal function. Currently, one of most promising exogenous markers GFR assessment iohexol. In this study, suitability volumetric absorptive microsampling (VAMS) as alternative conventional blood sampling and quantification iohexol in paediatric plasma was assessed. Therefore, a new, fully validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method developed. Subsequently, clinical evaluated 20 patients by comparing concentrations associated values obtained VAMS with those plasma. developed, simple cost-effective LC-MS/MS-method fulfilled all pre-set validation acceptance criteria. Iohexol could be accurately quantified within haematocrit range 20–60% long-term stability demonstrated up to 245 days under different storage temperatures. Both (r = 0.98, mean bias: −4.20%) derived 0.99; 1.31%), method, good correlation acceptable bias. agreement between two methods especially higher than 60 mL/min/1.73 m2. Nevertheless, <60 m2 accuracy compared lower. However, small adjustments protocol probably solve problem.